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[Music]

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I'm gonna run you through a little bit

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different scenario than we've had in

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this convention so far but just give an

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idea of what we're about we have a

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technology platform that we call

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resonant molecular signaling and it

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ought to resonate with this group we're

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convinced that good health depends on

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typical communication between and

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amongst within and among cells any

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disruption of that signaling will result

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in disease it can be acute like a stroke

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or chronic like rheumatoid arthritis our

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goal using resident molecular signaling

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is simply to use molecules and

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oftentimes fractions of molecules to

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restore communication and let the body

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do what it's supposed to do the

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therapeutic agents that we use in

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Resident alekhya leur signaling are as a

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group selected after studying the

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pathophysiology of disease what we're

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interested in is we we look at a disease

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disease X whatever it might be look at

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the sequence of events leading from

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initiation of disease to occurrence of

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signs and symptoms and see where we can

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interfere with that then think about a

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molecule that might be able to do that

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interference and we come up with these

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molecules either just brainstorming

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maybe reading the literature or just by

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trial and error with one exception all

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the molecules we use therapeutically are

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naturally occurring they are often well

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studied but also with but one exception

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they've never been used therapeutically

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before so they're all together different

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than what's on the market now they are

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safe and we have done I think we're into

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our sixteenth phase to FDA trial we've

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done a couple USDA trials also in all of

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those trials to put the safety profile

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for the placebo placebo group is the

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same as for the treated group so these

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things are safe very interesting is they

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are stable at all these different

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temperatures that's interesting because

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some of these molecules are enzymes or

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hormones and those of you that work in

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biology know that those are not stable

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at room temperature or at pocket

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temperature things like that they are

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not stable with respect to their

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enzymatic or hormonal activity but

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that's not what we're looking at we're

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looking at a different kind of

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of activity and we administer these in

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what we call physiologic doses much more

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concentrated than a remedy that would be

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associated with homeopathy but less

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concentrated there might be a normal

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physiologic rub allopathic dose we're

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convinced that these agents that we use

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act as nothing more than signals to

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stimulate a cascade of events that

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results in the desired outcome of

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interest is there's no relationship

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between patient mass and the dose that

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we use so a 2,000 pound horse gets the

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same dose as a 5 pound chihuahua or 150

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pound man the frequency of dosing can

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change but never the dose itself also of

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interest is we can use different routes

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of administration with the same dose get

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the same response in the same amount of

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time you can't think of very many

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medicines that do that that again give

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us a hint that these are simply acting

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as signals what I want to do today is

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focus on just one of these therapeutic

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agents as kind of the prototype for our

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platform and this is streptomycin oh

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that's scribble on the right is supposed

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to resemble three-dimensional structure

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of streptomycin though I don't know if

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it does or not I've never seen it but

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that's what comes out of the literature

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this is a molecule that you're all

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familiar with one way or another this is

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the toxin from group a beta hemolytic

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streptococcus or throat and this is the

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toxin that causes a sore throat we use

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it as a therapeutic agent in a little

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bit a little bit different situation in

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that we modify the toxin we reduce it

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it's toxic in its I'm sorry it's toxic

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in its reduced state we modify it to the

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oxidized state it loses its toxicity and

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gains therapeutic activity of a

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different way that's the modification

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well there we go now one might wonder

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what the heck why would we ever use

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streptomycin o as a therapeutic agent in

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the first place there was a paper

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presented oh gosh she's gotta be eight

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or nine years ago now by a professor at

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Brown University at the Medical School

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and this gal did some epidemiological

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work to show that movement disorders

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like Tourette's and ticks and things

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like that

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in adults could be related to a

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childhood history of chronic or repeated

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strep infections we wondered what the

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heck could that relationship be that is

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clearly the organism was no longer there

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in the adult and yet we had these

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symptoms what might be happening and we

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thought there might be a situation

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involved with an aberrant immune

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response to the original infection that

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persisted through our life and could

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cause these problems so what we did was

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obtained some very very highly purified

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strep to licen though we oxidized it

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that's the modification step then we had

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to find a patient with this kind of a

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problem and we found one of a patient

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that was in the the practice of one of

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the physicians in our group who had a

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horrible tick of the neck and head she

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was always going like this about every 6

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or 7 seconds if you sat in the

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examination room with her within three

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or four minutes you're going like this -

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everybody's doing this she was 34 years

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old at the at the time of initiation of

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therapy she was being treated by this

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physician for another problem but he

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contacted her and said listen Mary would

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you be interested in trying this kind of

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left-wing wacko approach to seeing if we

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could do anything with your tip we're

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confident it'll be safe we haven't the

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foggiest idea if it's gonna do you any

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good

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she said yep let's give it a try so she

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came to the clinic she was instructed to

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take one drop under her tongue of this

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modified strep - Lysa no four times a

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day come back in four weeks she did that

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and at the end of the four weeks she

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came back and clearly her tick was

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reduced but it was not gone the

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physician had the good sense to say well

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gosh Mary did you see anything else good

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or bad that occurred during this four a

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week period and she goes like this

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William just about pushes the guy out

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she said look at this well yeah close up

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and I said it very nice looking fist

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what am I looking at and she said well

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when I was eight or nine years old I was

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in my mother's kitchen and I pulled a

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sauce pan of boiling water off the stove

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onto my hand and I burned my knuckles

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very badly scarred we thought

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permanently and in four weeks those

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scars are gone well

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that's kind of interesting how do you

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makes cars go you're taking a drop out

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of the tongue and this cows cars

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disappear so we thought well geez oh man

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that might be kind of interesting all by

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itself and we brought other patients in

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who had superficial scars of this sort

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that we could see and we found that yes

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indeed without exception we could

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certainly reduce the scars and sometimes

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make them go away completely in a

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four-week period again taking sublingual

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drops and it didn't matter where the

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scars were as we got more and more into

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this we found that the spectrum of

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targets for SLO therapy expanded far

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beyond scars and we'll take a look at

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some of that you know a little bit later

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today but wanted to give you an idea of

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some of the problems that we had trying

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to get our heads around what the heck's

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going on here we said what does SLO do

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this little photo micrograph collection

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is from collaborative work we did with

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investigators at NYU and what we have

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here whoops

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whichever hand has the pointer what we

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have here is human tissue grown on a

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microscope slide we cut the tissue was

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with a scalpel and then you'll see that

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we've used two different concentrations

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of slo exposed some cells that is some

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microscope some cells on microscope

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flies to SLO and have controls here and

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what we're measuring is the rate at

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which the gap closes this is superficial

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healing and what we found was that there

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is an incredibly increased rate of

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activation mobilization of character

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sites that causes closing the gap much

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faster than what happened under normal

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conditions well great let's go a little

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bit deeper in that and see what genes

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are up and downregulated is a

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consequence of SLO exposure and we're

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certainly not going to go through all

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this but there are a couple important

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things here that you maybe heard of cd44

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antigen especially important in aligning

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collagen in proper sequence for scar

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formation for wound repair and so forth

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and we are upregulating that that's an

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anti inflammatory cytokine also so

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we're getting anti-inflammatory effects

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from SLO this one some of you might even

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take chondroitin nutritional supplement

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if you have arthritis in your knee or a

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sore elbow chondroitin is a typical

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nutritional supplement for that we think

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it's better to induce the body to

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generate its own and we can do it with

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SLO and a couple other molecules we use

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but complementary complementary Carolee

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to the upregulation in these

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anti-inflammatory cytokines was the down

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regulation of pro-inflammatory cytokines

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extremely important especially this guy

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which probably most of you have never

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heard of the matrix metalloproteinases

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these are collective group of enzymes

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that are implicated in a variety of

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diseases ranging and this is not being

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flippant about it

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ranging from sore throats to cancer

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these are really really dynamic proteins

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and when they are up regulated there's

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nothing but trouble the pharmaceutical

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industry over the last eight years has

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spent over six billion dollars to find

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inhibitors of

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mmp9 and MMP - they often go hand in

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hand - is not on this list because

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although we down regulated it in this

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experiment it wasn't to a statistically

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significant level the point is that we

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are simultaneously up regulating anti

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inflammatory cytokines down-regulating

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pro-inflammatory cytokines and

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down-regulating mmp-9 and we'll see what

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that means here a little while so let's

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look at some some practical applications

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of what we're finding with streptomycin

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though these four experiments are going

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to get about two minutes each these are

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all done in horses all done in with

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collaboration with investigators at the

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University of Kentucky or with

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practicing veterinarians and the large

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equine practices in the Lexington area

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all the animals are going to look at our

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extremely expensive thoroughbreds that

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have diseases for which there isn't much

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to offer first off the bat that's a

275
00:11:13,919 --> 00:11:11,259
horse's leg actually two legs of horses

276
00:11:16,199 --> 00:11:13,929
and what we're interested here is right

277
00:11:17,639 --> 00:11:16,209
here oftentimes a horse looking injured

278
00:11:24,929 --> 00:11:17,649
that

279
00:11:26,999 --> 00:11:24,939
horse limping and not being able to be

280
00:11:30,480 --> 00:11:27,009
athletic anymore is it the tendon will

281
00:11:32,759 --> 00:11:30,490
tighten up and the hoof will lose its

282
00:11:34,499 --> 00:11:32,769
normal orientation to the ground the

283
00:11:36,480 --> 00:11:34,509
horse will be unable to use that leg

284
00:11:38,189 --> 00:11:36,490
anymore it'll go down and it won't come

285
00:11:39,290 --> 00:11:38,199
back up that that'll be a horse that's

286
00:11:42,150 --> 00:11:39,300
going to be put to sleep

287
00:11:44,519 --> 00:11:42,160
what is done for therapy for this often

288
00:11:46,559 --> 00:11:44,529
times is to cut this tendon and what

289
00:11:48,150 --> 00:11:46,569
that does is release the pressure that's

290
00:11:49,889 --> 00:11:48,160
occurring as a result of the tightening

291
00:11:51,900 --> 00:11:49,899
after the injury scar tissue forms

292
00:11:54,449 --> 00:11:51,910
there's a tightening as cross linkage of

293
00:11:56,040 --> 00:11:54,459
collagen occurs if the tendon becomes

294
00:11:59,669 --> 00:11:56,050
less flexible now we're going to cut it

295
00:12:02,460 --> 00:11:59,679
that allows the hoof to regain its

296
00:12:03,600 --> 00:12:02,470
orientation a little bit better but what

297
00:12:05,249 --> 00:12:03,610
happens when you do something like that

298
00:12:10,259 --> 00:12:05,259
you actually generate scar tissue also

299
00:12:11,519 --> 00:12:10,269
and so we have a tenotomy that's the

300
00:12:15,629 --> 00:12:11,529
cutting of the tendon that's going to

301
00:12:18,689 --> 00:12:15,639
occur with this with this situation what

302
00:12:20,639 --> 00:12:18,699
we want to do is decrease or eliminate

303
00:12:21,929 --> 00:12:20,649
the scar tissue that forms after the

304
00:12:25,139 --> 00:12:21,939
tenotomy and we're going to do that

305
00:12:27,809 --> 00:12:25,149
using slo which we find alters the ratio

306
00:12:30,150 --> 00:12:27,819
of collagen to collagenase collagenous

307
00:12:32,429 --> 00:12:30,160
breaks down collagenase breaks down

308
00:12:34,410 --> 00:12:32,439
collagen allows it to be replaced by

309
00:12:35,429 --> 00:12:34,420
more vital tissue and here's what we

310
00:12:37,379 --> 00:12:35,439
have and we're certainly not going to go

311
00:12:39,299 --> 00:12:37,389
through all this stuff except look at

312
00:12:42,480 --> 00:12:39,309
the pretty colors what we're after in

313
00:12:45,210 --> 00:12:42,490
this is we would like to see the dark

314
00:12:48,119 --> 00:12:45,220
the dark purple being shorter than the

315
00:12:51,119 --> 00:12:48,129
the light purple or the blue and this

316
00:12:53,100 --> 00:12:51,129
looks at post versus pre and what we're

317
00:12:55,350 --> 00:12:53,110
doing here is sonography where we're

318
00:12:57,389 --> 00:12:55,360
using a sonogram on the area of where

319
00:12:59,309 --> 00:12:57,399
the tenotomy is done so we get quite

320
00:13:01,259 --> 00:12:59,319
objective measurements of the amount of

321
00:13:02,910 --> 00:13:01,269
scar tissue by looking at three

322
00:13:04,590 --> 00:13:02,920
dimensional cross-sections of the tissue

323
00:13:07,199 --> 00:13:04,600
it all comes out that is the

324
00:13:09,960 --> 00:13:07,209
measurements come out of the computer so

325
00:13:13,499 --> 00:13:09,970
again its objective and what we want to

326
00:13:17,489 --> 00:13:13,509
have is smaller cross-sectional areas in

327
00:13:18,809 --> 00:13:17,499
the post this color versus the pre here

328
00:13:20,730 --> 00:13:18,819
looks pretty good this is after 30 days

329
00:13:23,369 --> 00:13:20,740
treatment that looks pretty good that's

330
00:13:25,139 --> 00:13:23,379
a turkey that's exactly wrong what went

331
00:13:27,569 --> 00:13:25,149
wrong dumb horse stepped in a hole

332
00:13:31,170 --> 00:13:27,579
during the four weeks of therapy and

333
00:13:33,810 --> 00:13:31,180
re-injured itself that's the way it goes

334
00:13:36,060 --> 00:13:33,820
this is a kind of a fun one in that you

335
00:13:37,920 --> 00:13:36,070
can see we're gaining a lot here we're

336
00:13:40,110 --> 00:13:37,930
starting to see something here after 30

337
00:13:42,720 --> 00:13:40,120
days the importance is this

338
00:13:45,870 --> 00:13:42,730
animal-headed tenotomy seven years ago

339
00:13:47,820 --> 00:13:45,880
that's an old old scar and to get any

340
00:13:50,370 --> 00:13:47,830
resolution or a hint of resolution after

341
00:13:51,810 --> 00:13:50,380
that period is something that is fairly

342
00:13:53,160 --> 00:13:51,820
unheard of and you can see the stats

343
00:13:56,100 --> 00:13:53,170
here get fifty to thirty percent

344
00:13:58,490 --> 00:13:56,110
resolution in 30 days that's a start on

345
00:14:01,410 --> 00:13:58,500
something that could be quite useful

346
00:14:02,730 --> 00:14:01,420
another problem with horses again for

347
00:14:04,740 --> 00:14:02,740
which there's little therapy especially

348
00:14:07,710 --> 00:14:04,750
in these thoroughbreds it's a problem

349
00:14:09,990 --> 00:14:07,720
our cervical adhesions these adhesions

350
00:14:13,560 --> 00:14:10,000
occur after especially multiple births

351
00:14:16,560 --> 00:14:13,570
just the act of birthing results in scar

352
00:14:18,900 --> 00:14:16,570
tissue formation and what we have here

353
00:14:20,610 --> 00:14:18,910
are eleven mares these are all

354
00:14:23,130 --> 00:14:20,620
high-price there are bred mayors that

355
00:14:26,940 --> 00:14:23,140
are barren which means that they are

356
00:14:30,000 --> 00:14:26,950
really only good for dog food anymore we

357
00:14:33,180 --> 00:14:30,010
have a an accepted these are called

358
00:14:36,410 --> 00:14:33,190
Kinney scores here we've done biopsies

359
00:14:39,390 --> 00:14:36,420
prior to SLO therapy of the cervical

360
00:14:41,370 --> 00:14:39,400
region of the horse and the Kimi scores

361
00:14:43,110 --> 00:14:41,380
go from one to three and there's a to go

362
00:14:45,180 --> 00:14:43,120
to to be you'll see in here the higher

363
00:14:47,130 --> 00:14:45,190
the number the more seriously afflicted

364
00:14:49,260 --> 00:14:47,140
the horse here we have the years which

365
00:14:51,720 --> 00:14:49,270
the mirrors have been barren here's the

366
00:14:54,720 --> 00:14:51,730
expected Foley rate or the following

367
00:14:57,270 --> 00:14:54,730
percentage 38% look at this you get

368
00:14:58,920 --> 00:14:57,280
something like number 10 has a kidney

369
00:15:00,570 --> 00:14:58,930
score of 3 that's as bad as it gets it's

370
00:15:03,780 --> 00:15:00,580
barren for a year it only has a 10%

371
00:15:07,560 --> 00:15:03,790
chance of ever having a live full okay

372
00:15:09,570 --> 00:15:07,570
this is a $500,000 mayor and she's not a

373
00:15:11,910 --> 00:15:09,580
racing mare she's a brood mare and she's

374
00:15:16,770 --> 00:15:11,920
not worth much if she can't have a foal

375
00:15:19,890 --> 00:15:16,780
so after 30 days of therapy with SLO she

376
00:15:22,020 --> 00:15:19,900
was bred the breeding was successful the

377
00:15:24,780 --> 00:15:22,030
fetus was maintained she had a live

378
00:15:27,300 --> 00:15:24,790
birth always well so bottom line is

379
00:15:29,880 --> 00:15:27,310
right down there in the corner

380
00:15:31,440 --> 00:15:29,890
we had a know just a dinky little study

381
00:15:34,470 --> 00:15:31,450
but it looks like SLO is doing something

382
00:15:36,330 --> 00:15:34,480
and remember this SLO is being

383
00:15:38,820 --> 00:15:36,340
introduced in these cases by a tiny

384
00:15:40,770 --> 00:15:38,830
injection in the neck to heal scarring

385
00:15:43,200 --> 00:15:40,780
in the uterus or in the cervix okay so

386
00:15:44,820 --> 00:15:43,210
we're getting a systemic reaction that

387
00:15:46,230 --> 00:15:44,830
way

388
00:15:48,150 --> 00:15:46,240
this is where it really gets exciting no

389
00:15:49,590 --> 00:15:48,160
this is kind of fun we're gonna look at

390
00:15:52,730 --> 00:15:49,600
a marriage uterus don't blush

391
00:15:55,400 --> 00:15:52,740
there's nothing pornographic about this

392
00:16:00,450 --> 00:15:55,410
what we're going to look at is this is a

393
00:16:03,660 --> 00:16:00,460
$750,000 mayor that had a had a Colt a

394
00:16:07,590 --> 00:16:03,670
good healthy Colt and after giving birth

395
00:16:09,240 --> 00:16:07,600
had a horrible uterine infection so as

396
00:16:11,550 --> 00:16:09,250
is typical for treating that sort of a

397
00:16:14,220 --> 00:16:11,560
case the the uterus was drenched with

398
00:16:15,540 --> 00:16:14,230
antibiotic the problem was the attending

399
00:16:17,760 --> 00:16:15,550
veterinarian didn't realize the horse

400
00:16:19,950 --> 00:16:17,770
was allergic to that antibiotic so there

401
00:16:22,650 --> 00:16:19,960
was a terrible allergic reaction

402
00:16:25,320 --> 00:16:22,660
inflammatory reaction in this uterus as

403
00:16:30,480 --> 00:16:25,330
a consequence of therapy now what we're

404
00:16:33,810 --> 00:16:30,490
going to do if I can remember how to do

405
00:16:36,180 --> 00:16:33,820
it is this we have a camera inside the

406
00:16:38,340 --> 00:16:36,190
uterus and I know this isn't the sort of

407
00:16:39,840 --> 00:16:38,350
thing you pay big bucks for but what

408
00:16:42,780 --> 00:16:39,850
we're looking at is the degree of

409
00:16:44,280 --> 00:16:42,790
scarring in this uterus and we're gonna

410
00:16:47,960 --> 00:16:44,290
see in a second this thing only lasts

411
00:16:51,210 --> 00:16:47,970
for twenty or thirty seconds look those

412
00:16:53,220 --> 00:16:51,220
those strands in there that are white

413
00:16:54,660 --> 00:16:53,230
that look some of them we'll see you

414
00:16:56,310 --> 00:16:54,670
kind of look like stalactites and

415
00:16:58,200 --> 00:16:56,320
stalagmites and they form columns that's

416
00:17:00,900 --> 00:16:58,210
all connective tissue and there's no way

417
00:17:03,720 --> 00:17:00,910
on earth that this mayor is ever going

418
00:17:07,230 --> 00:17:03,730
to be able to maintain a fetus with a

419
00:17:10,980 --> 00:17:07,240
uterus like that so we treated her with

420
00:17:12,720 --> 00:17:10,990
slo for 35 45 days she was bred

421
00:17:15,390 --> 00:17:12,730
successfully and she's had three live

422
00:17:18,449 --> 00:17:15,400
foals since that one treatment so again

423
00:17:20,550 --> 00:17:18,459
that's a that's a big deal in the horse

424
00:17:22,079 --> 00:17:20,560
industry the last thing to talk about

425
00:17:24,630 --> 00:17:22,089
horses is the last thing we want to look

426
00:17:26,630 --> 00:17:24,640
at is a recently completed study we did

427
00:17:29,670 --> 00:17:26,640
at the University of Kentucky

428
00:17:33,270 --> 00:17:29,680
Rhodococcus equi is a soil borne

429
00:17:34,320 --> 00:17:33,280
bacterium that infects young foals when

430
00:17:35,970 --> 00:17:34,330
they're out in the pasture kicking

431
00:17:37,920 --> 00:17:35,980
around and learning how to walk and

432
00:17:39,810 --> 00:17:37,930
things like that they breathe in dust

433
00:17:41,880 --> 00:17:39,820
that's contaminated with this organism

434
00:17:43,170 --> 00:17:41,890
and they get pneumonia and pneumonia can

435
00:17:48,120 --> 00:17:43,180
be fatal or it can be chronic

436
00:17:54,180 --> 00:17:48,130
what we did was artificially infect some

437
00:17:56,190 --> 00:17:54,190
foals with Rhodococcus equi and we

438
00:17:58,620 --> 00:17:56,200
divide them then into four groups those

439
00:18:01,080 --> 00:17:58,630
treated with just saline those treated

440
00:18:02,910 --> 00:18:01,090
SLO alone with the typical antibiotic

441
00:18:04,830 --> 00:18:02,920
therapy alone and the combination of

442
00:18:07,980 --> 00:18:04,840
antibiotics that's what the a B stands

443
00:18:11,490 --> 00:18:07,990
for and SLO now the reason for this is

444
00:18:12,510 --> 00:18:11,500
that in the usual route of in federal

445
00:18:14,760 --> 00:18:12,520
consequential infection the

446
00:18:15,960 --> 00:18:14,770
pathophysiology of this disease is that

447
00:18:18,270 --> 00:18:15,970
the organism gets in the lung of the

448
00:18:20,880 --> 00:18:18,280
horses it can cause immediate damage

449
00:18:22,740 --> 00:18:20,890
there to the point of fatality for the

450
00:18:24,420 --> 00:18:22,750
young foal or it can cause a chronic

451
00:18:27,030 --> 00:18:24,430
condition regardless of which it does

452
00:18:28,860 --> 00:18:27,040
well that's if there's if the foal

453
00:18:30,180 --> 00:18:28,870
survives it's going to be compromised

454
00:18:33,000 --> 00:18:30,190
the rest of its life because of a

455
00:18:34,830 --> 00:18:33,010
reduced respiratory capacity also of

456
00:18:37,860 --> 00:18:34,840
interest is that the organism becomes

457
00:18:39,540 --> 00:18:37,870
encapsulated within the lung later in

458
00:18:41,310 --> 00:18:39,550
life that capsule can breakdown live

459
00:18:43,860 --> 00:18:41,320
organisms can be spewed out underneath

460
00:18:45,750 --> 00:18:43,870
any time infection can occur again the

461
00:18:47,250 --> 00:18:45,760
reason we wanted to try slo in

462
00:18:48,600 --> 00:18:47,260
combination with the antibiotics was to

463
00:18:51,090 --> 00:18:48,610
prevent that encapsulation

464
00:18:52,680 --> 00:18:51,100
because if encapsulation occurs the

465
00:18:53,940 --> 00:18:52,690
antibiotic can never reach the organism

466
00:18:56,670 --> 00:18:53,950
so you can't kill it

467
00:18:58,920 --> 00:18:56,680
so those are the results of this little

468
00:19:00,480 --> 00:18:58,930
experiment the clinical score is a

469
00:19:02,040 --> 00:19:00,490
determination made by the attending

470
00:19:04,410 --> 00:19:02,050
veterinarian who's blinded as to which

471
00:19:07,170 --> 00:19:04,420
horse is in which of the four groups I

472
00:19:10,110 --> 00:19:07,180
mentioned and we got a p-value not bad

473
00:19:11,760 --> 00:19:10,120
of O 5 in the treated animals that is

474
00:19:13,560 --> 00:19:11,770
treated with the antibiotic process

475
00:19:16,860 --> 00:19:13,570
alone we're better off than those with

476
00:19:18,990 --> 00:19:16,870
antibiotic alone also we saw the 30 plus

477
00:19:21,440 --> 00:19:19,000
percent reduction in pneumonia scores in

478
00:19:23,700 --> 00:19:21,450
the combination another nice p-value but

479
00:19:26,190 --> 00:19:23,710
whoops wrong hand look at this guy down

480
00:19:29,280 --> 00:19:26,200
here this is really interesting here

481
00:19:30,630 --> 00:19:29,290
we're getting less collagen and that

482
00:19:32,610 --> 00:19:30,640
remember that's going to be the primary

483
00:19:35,670 --> 00:19:32,620
component in the capsulation process

484
00:19:36,870 --> 00:19:35,680
down here so that the organism that is

485
00:19:41,130 --> 00:19:36,880
causing the problem is going to be

486
00:19:45,060 --> 00:19:41,140
exposed more efficiently to the

487
00:19:47,520 --> 00:19:45,070
antibiotics and a nice p-value so what

488
00:19:50,280 --> 00:19:47,530
who cares about all these horses well we

489
00:19:53,820 --> 00:19:50,290
do in part because this is a recognized

490
00:19:55,850 --> 00:19:53,830
model for that TB in humans tuberculosis

491
00:19:57,990 --> 00:19:55,860
and humans has the same situation

492
00:19:59,580 --> 00:19:58,000
breathing the organism it can become

493
00:20:00,890 --> 00:19:59,590
encapsulated those capsules are called

494
00:20:04,440 --> 00:20:00,900
tube records you can pick them up on

495
00:20:05,400 --> 00:20:04,450
chest x-rays in this country though we

496
00:20:07,620 --> 00:20:05,410
don't get really excited about

497
00:20:10,230 --> 00:20:07,630
tuberculosis anymore there are between

498
00:20:12,169 --> 00:20:10,240
50 and 55 thousand new cases per year

499
00:20:13,820 --> 00:20:12,179
worldwide

500
00:20:16,519 --> 00:20:13,830
or not one-third of the world's

501
00:20:18,830 --> 00:20:16,529
population has tuberculosis that's a

502
00:20:21,470 --> 00:20:18,840
pretty big bunch now some of these

503
00:20:23,299 --> 00:20:21,480
people that have it are going to suffer

504
00:20:25,159 --> 00:20:23,309
the acute infection and die early on a

505
00:20:27,799 --> 00:20:25,169
lot of for a lot of people it's going to

506
00:20:30,470 --> 00:20:27,809
be a chronic infection that might become

507
00:20:31,850 --> 00:20:30,480
acute at any time as a result of

508
00:20:34,070 --> 00:20:31,860
breaking down the capsule or the

509
00:20:36,919 --> 00:20:34,080
tubercle in this case to release the

510
00:20:40,279 --> 00:20:36,929
organism we have a study planned in

511
00:20:41,450 --> 00:20:40,289
India where we will do in people that

512
00:20:44,090 --> 00:20:41,460
are afflicted with Mycobacterium

513
00:20:45,230 --> 00:20:44,100
tuberculosis the cause evasion we're

514
00:20:47,600 --> 00:20:45,240
going to do the same experiment that we

515
00:20:51,499 --> 00:20:47,610
did in the horses in Kentucky we are

516
00:20:54,499 --> 00:20:51,509
going to combine antibiotics with slo to

517
00:20:56,480 --> 00:20:54,509
see if we can break down the the capsule

518
00:20:58,310 --> 00:20:56,490
it's forming the typical expose the

519
00:20:59,239 --> 00:20:58,320
organism to the antibiotic and clear the

520
00:21:01,789 --> 00:20:59,249
people so that they're no longer

521
00:21:05,210 --> 00:21:01,799
chronically infected the importance of

522
00:21:07,999 --> 00:21:05,220
that is twofold one is typical

523
00:21:11,570 --> 00:21:08,009
antibiotic therapy for TB now lasts six

524
00:21:13,789 --> 00:21:11,580
months it's hard to keep anybody on any

525
00:21:15,859 --> 00:21:13,799
regimen for six months so people are

526
00:21:18,499 --> 00:21:15,869
going to be chronic carriers for a long

527
00:21:20,299 --> 00:21:18,509
time second thing is the longer an

528
00:21:22,639 --> 00:21:20,309
organism exposed is exposed to an

529
00:21:24,499 --> 00:21:22,649
antibiotic the greater the chance of an

530
00:21:26,899 --> 00:21:24,509
antibiotic resistant strain developing

531
00:21:29,060 --> 00:21:26,909
so what we want to do is see if we can

532
00:21:33,320 --> 00:21:29,070
reduce the clinical time of treatment

533
00:21:35,060 --> 00:21:33,330
for tuberculosis from six months to one

534
00:21:37,070 --> 00:21:35,070
or two months just doing that

535
00:21:40,070 --> 00:21:37,080
understanding that slo has no

536
00:21:41,509 --> 00:21:40,080
antimicrobial activity of its own it's

537
00:21:43,129 --> 00:21:41,519
got to be used in conjunction with the

538
00:21:46,669 --> 00:21:43,139
antibiotics but we think we might be

539
00:21:49,039 --> 00:21:46,679
able to help now we've talked about some

540
00:21:50,600 --> 00:21:49,049
internal scarring associated diseases

541
00:21:52,399 --> 00:21:50,610
with horses we looked at the legs of the

542
00:21:55,549 --> 00:21:52,409
one horse on the uterus and the cervix

543
00:21:58,609 --> 00:21:55,559
and so forth what about humans these

544
00:21:59,989 --> 00:21:58,619
first four dot points represent some of

545
00:22:03,289 --> 00:21:59,999
the diseases we've treated successfully

546
00:22:06,350 --> 00:22:03,299
using slo these are all sky related

547
00:22:08,090 --> 00:22:06,360
diseases cardiomyopathy horrible disease

548
00:22:10,310 --> 00:22:08,100
we've had a couple patients that were on

549
00:22:12,529 --> 00:22:10,320
the cardiac transplant list that are no

550
00:22:14,899 --> 00:22:12,539
longer on it so that's that's a good

551
00:22:17,060 --> 00:22:14,909
sign polycystic kidney disease no

552
00:22:18,649 --> 00:22:17,070
responds quickly surgical adhesions are

553
00:22:21,139 --> 00:22:18,659
a big problem especially with gut

554
00:22:23,090 --> 00:22:21,149
surgery or gynecological surgery we can

555
00:22:25,220 --> 00:22:23,100
make those surgical adhesions pretty

556
00:22:28,670 --> 00:22:25,230
much disappear

557
00:22:31,160 --> 00:22:28,680
the range of motion for all of us

558
00:22:34,100 --> 00:22:31,170
decreases with age in part because of

559
00:22:35,690 --> 00:22:34,110
adhesions and we have little study

560
00:22:37,070 --> 00:22:35,700
actually a pretty good size study ready

561
00:22:38,780 --> 00:22:37,080
to start later this year at Yale

562
00:22:40,310 --> 00:22:38,790
University Medical School where we will

563
00:22:42,260 --> 00:22:40,320
treat patients with complications of

564
00:22:43,700 --> 00:22:42,270
total knee replacement surgery because

565
00:22:45,350 --> 00:22:43,710
anecdotally we've seen these patients

566
00:22:48,410 --> 00:22:45,360
get a heck of a lot better with slo

567
00:22:50,060 --> 00:22:48,420
therapy we are just now starting to put

568
00:22:52,180 --> 00:22:50,070
our foot in the water with respect to

569
00:22:54,590 --> 00:22:52,190
treating concussions

570
00:22:55,850 --> 00:22:54,600
we're not scarring in the brain is a

571
00:22:57,320 --> 00:22:55,860
little bit different than it then

572
00:22:58,550 --> 00:22:57,330
scarring elsewhere in the body we're not

573
00:23:00,800 --> 00:22:58,560
sure what will happen here but we're

574
00:23:03,650 --> 00:23:00,810
working with two groups on this project

575
00:23:05,500 --> 00:23:03,660
this proposed project one is the with

576
00:23:07,880 --> 00:23:05,510
the people at the Medical School in

577
00:23:09,560 --> 00:23:07,890
Johnson City Tennessee eastern Tennessee

578
00:23:11,930 --> 00:23:09,570
Medical School the other group is called

579
00:23:14,570 --> 00:23:11,940
the National Football League and we're

580
00:23:19,750 --> 00:23:14,580
going to see if if SLO can do anything

581
00:23:25,100 --> 00:23:23,180
we've talked a little bit about external

582
00:23:26,720 --> 00:23:25,110
scars in humans one of the first

583
00:23:29,840 --> 00:23:26,730
patients was well maybe I didn't tell

584
00:23:31,790 --> 00:23:29,850
you about that I told you about the gal

585
00:23:33,920 --> 00:23:31,800
that tried to punch out to the physician

586
00:23:37,340 --> 00:23:33,930
one of the other patients we brought in

587
00:23:41,170 --> 00:23:37,350
was a man in his in his mid-70s 75 76

588
00:23:44,600 --> 00:23:41,180
years old who had acne scars since since

589
00:23:47,450 --> 00:23:44,610
his teenage years and in four weeks

590
00:23:49,820 --> 00:23:47,460
those flattened out okay so this this is

591
00:23:51,320 --> 00:23:49,830
a hint of another use for this and prom

592
00:23:56,030 --> 00:23:51,330
and do scars stretch marks and all that

593
00:24:00,430 --> 00:23:56,040
that's great what about if we used slo

594
00:24:04,370 --> 00:24:00,440
to prevent scars rather than to just

595
00:24:06,200 --> 00:24:04,380
treat them well this is a picture of a

596
00:24:06,770 --> 00:24:06,210
gals face just after she went through a

597
00:24:10,220 --> 00:24:06,780
windshield

598
00:24:12,290 --> 00:24:10,230
there's her chin here's her teeth her

599
00:24:16,070 --> 00:24:12,300
nose has been torn off and displaced to

600
00:24:17,780 --> 00:24:16,080
the right she's in trouble at the time

601
00:24:19,340 --> 00:24:17,790
of the picture she's at the emergency

602
00:24:23,720 --> 00:24:19,350
room at the Medical University of South

603
00:24:24,830 --> 00:24:23,730
Carolina she's getting sewed up here you

604
00:24:26,660 --> 00:24:24,840
can see there gonna be a problem with

605
00:24:28,640 --> 00:24:26,670
the scar here look at the position this

606
00:24:29,360 --> 00:24:28,650
tube the right side of her mouth has

607
00:24:31,850 --> 00:24:29,370
been ripped out

608
00:24:33,830 --> 00:24:31,860
they got her nose back on now but there

609
00:24:36,200 --> 00:24:33,840
gonna be some scarring there some scar

610
00:24:38,270 --> 00:24:36,210
over the eyes and so forth her mother is

611
00:24:39,020 --> 00:24:38,280
a nurse and one of the practices that's

612
00:24:41,540 --> 00:24:39,030
in our group

613
00:24:43,310 --> 00:24:41,550
when she got the call from the from the

614
00:24:44,570 --> 00:24:43,320
medical school saying that her daughter

615
00:24:46,040 --> 00:24:44,580
had been the accident she ran out the

616
00:24:49,760 --> 00:24:46,050
door and said can I take some SLO with

617
00:24:52,430 --> 00:24:49,770
me and of course she did and this

618
00:24:54,560 --> 00:24:52,440
patient then started SLO again just a

619
00:24:57,890 --> 00:24:54,570
drop under the tongue four times a day

620
00:25:01,280 --> 00:24:57,900
the day of her accident whoops wrong

621
00:25:02,540 --> 00:25:01,290
button here she is looking pretty great

622
00:25:04,520 --> 00:25:02,550
don't you think it looks like she might

623
00:25:07,550 --> 00:25:04,530
have a scar on her left cheek they're

624
00:25:08,120 --> 00:25:07,560
gonna get a scar on her nose she's in

625
00:25:12,170 --> 00:25:08,130
trouble

626
00:25:15,260 --> 00:25:12,180
there she is three weeks later and if

627
00:25:16,430 --> 00:25:15,270
you look she still has a scar something

628
00:25:17,630 --> 00:25:16,440
that by the way all these pictures are

629
00:25:20,630 --> 00:25:17,640
taken by her mother who's not a

630
00:25:23,270 --> 00:25:20,640
professional photographer there she is

631
00:25:25,340 --> 00:25:23,280
a month after after the last picture

632
00:25:26,690 --> 00:25:25,350
again that scar is still flattening out

633
00:25:28,430 --> 00:25:26,700
it's got a ways to go yet but that's not

634
00:25:30,470 --> 00:25:28,440
too bad you don't see much around the

635
00:25:33,950 --> 00:25:30,480
nose at all look at that that's coming

636
00:25:36,320 --> 00:25:33,960
right along this is a this is a step in

637
00:25:39,350 --> 00:25:36,330
the right direction now the physicians

638
00:25:41,180 --> 00:25:39,360
in our group use SLO routinely for

639
00:25:44,090 --> 00:25:41,190
patients before they go into therapy and

640
00:25:47,150 --> 00:25:44,100
what we have found is the patients heal

641
00:25:48,620 --> 00:25:47,160
with little or no scarring after I say

642
00:25:50,270 --> 00:25:48,630
after therapy after surgery before they

643
00:25:52,700 --> 00:25:50,280
go into surgery they're healing with

644
00:25:56,750 --> 00:25:52,710
little or no scarring they don't need as

645
00:25:58,580 --> 00:25:56,760
much analgesia after surgery and again

646
00:25:59,750 --> 00:25:58,590
we know some reason about that we saw

647
00:26:02,090 --> 00:25:59,760
the up regulation of the

648
00:26:04,550 --> 00:26:02,100
anti-inflammatory down regulation of

649
00:26:05,450 --> 00:26:04,560
pro-inflammatory cytokines and they heal

650
00:26:10,580 --> 00:26:05,460
much quicker and they hear without

651
00:26:12,530 --> 00:26:10,590
adhesions now now let's go back to this

652
00:26:14,180 --> 00:26:12,540
young lady for a second so I can

653
00:26:15,800 --> 00:26:14,190
introduce you that next slide this is

654
00:26:17,600 --> 00:26:15,810
all great stuff hooray you know great we

655
00:26:18,950 --> 00:26:17,610
can do something with scars but it still

656
00:26:22,550 --> 00:26:18,960
doesn't get to the point that we were

657
00:26:25,340 --> 00:26:22,560
asking at the very start of this tirade

658
00:26:27,080 --> 00:26:25,350
that I'm giving you that was we've

659
00:26:29,120 --> 00:26:27,090
mentioned that this platform the

660
00:26:31,640 --> 00:26:29,130
resident molecular signaling platform

661
00:26:33,260 --> 00:26:31,650
that we've developed is making an

662
00:26:35,600 --> 00:26:33,270
assumption that the therapeutic agents

663
00:26:38,300 --> 00:26:35,610
that we use are simply acting as signals

664
00:26:40,580 --> 00:26:38,310
and again if you can use the same

665
00:26:43,340 --> 00:26:40,590
product for a 2,000 pound horse as a you

666
00:26:44,570 --> 00:26:43,350
know as a cat and which we do good it

667
00:26:46,430 --> 00:26:44,580
works just like that

668
00:26:48,320 --> 00:26:46,440
it works super but what's the nature of

669
00:26:50,390 --> 00:26:48,330
the signal well we're not smart enough

670
00:26:52,730 --> 00:26:50,400
to figure it out ourselves so we asked

671
00:26:54,230 --> 00:26:52,740
the help of the personnel

672
00:26:56,419 --> 00:26:54,240
what's called the center for cell

673
00:26:57,710 --> 00:26:56,429
signaling at the medical medical school

674
00:27:00,230 --> 00:26:57,720
at the University of Virginia

675
00:27:02,720 --> 00:27:00,240
David Brautigan is the director of that

676
00:27:04,669 --> 00:27:02,730
program down there we got in touch with

677
00:27:06,380 --> 00:27:04,679
him send him some of the data and a

678
00:27:08,690 --> 00:27:06,390
whole lot more some of the data you've

679
00:27:11,240 --> 00:27:08,700
seen here and an awful lot more and said

680
00:27:13,159 --> 00:27:11,250
can you help us figure out what's going

681
00:27:14,389 --> 00:27:13,169
on here he looked at all the data he

682
00:27:16,669 --> 00:27:14,399
says I've never seen anything like it

683
00:27:18,440 --> 00:27:16,679
and this is the guy that's director of

684
00:27:20,720 --> 00:27:18,450
cell signaling say well can you help us

685
00:27:22,450 --> 00:27:20,730
anyway he said let's do this we've got

686
00:27:24,440 --> 00:27:22,460
some in vitro assays we do a lot of

687
00:27:26,029 --> 00:27:24,450
cancer work down here at University of

688
00:27:28,370 --> 00:27:26,039
Virginia we have some in vitro assays

689
00:27:30,320 --> 00:27:28,380
you'll recall one of the slides way back

690
00:27:34,070 --> 00:27:30,330
showed down-regulation of

691
00:27:35,840 --> 00:27:34,080
mmp9 that's one of the markers that's

692
00:27:38,180 --> 00:27:35,850
associated with cancer he said let's

693
00:27:41,090 --> 00:27:38,190
take a look since you've got this this

694
00:27:42,799 --> 00:27:41,100
date about mmp9 let's see if SLO does

695
00:27:44,269 --> 00:27:42,809
anything in one of our in vitro assays

696
00:27:46,639 --> 00:27:44,279
then that'll give us a handle on where

697
00:27:50,269 --> 00:27:46,649
to go next so now we get to look at this

698
00:27:51,350 --> 00:27:50,279
slide what we have here whoops in the in

699
00:27:52,190 --> 00:27:51,360
the top four little pictures are

700
00:27:56,690 --> 00:27:52,200
photomicrographs

701
00:28:00,830 --> 00:27:56,700
of cells that have passed through a gel

702
00:28:03,529 --> 00:28:00,840
matrix that that reflects the ability of

703
00:28:07,669 --> 00:28:03,539
those cells to metastasize in the human

704
00:28:11,210 --> 00:28:07,679
body these cells are of human breast

705
00:28:13,549 --> 00:28:11,220
cancer origin they are them the most

706
00:28:16,310 --> 00:28:13,559
aggressive form of breast cancer in

707
00:28:18,560 --> 00:28:16,320
humans okay now look all we're doing

708
00:28:19,820 --> 00:28:18,570
without getting into too much detail is

709
00:28:20,960 --> 00:28:19,830
counting the number of cells that have

710
00:28:22,789 --> 00:28:20,970
made it through the matrix that's an

711
00:28:25,010 --> 00:28:22,799
indication of their degree of metastatic

712
00:28:27,380 --> 00:28:25,020
ability okay and you look at different

713
00:28:29,269 --> 00:28:27,390
concentrations of slo and you have

714
00:28:32,480 --> 00:28:29,279
progressively fewer cells making it

715
00:28:34,250 --> 00:28:32,490
through these bar graphs simply show in

716
00:28:36,970 --> 00:28:34,260
in this one what we're seeing in these

717
00:28:40,430 --> 00:28:36,980
pictures okay and each one of these is

718
00:28:42,350 --> 00:28:40,440
statistically significant so now we have

719
00:28:43,610 --> 00:28:42,360
a product that not only does something

720
00:28:44,419 --> 00:28:43,620
with scars that looks like it emits

721
00:28:47,600 --> 00:28:44,429
metastases

722
00:28:49,399 --> 00:28:47,610
when dr. Brautigan uva saw this the

723
00:28:52,639 --> 00:28:49,409
first thing he said was every woman over

724
00:28:54,830 --> 00:28:52,649
40 should be taking us hello that that's

725
00:28:56,779 --> 00:28:54,840
and this this is the guy that works with

726
00:29:00,169 --> 00:28:56,789
cancer all the time well maybe yes maybe

727
00:29:01,730 --> 00:29:00,179
no the point of it is that we are now in

728
00:29:04,899 --> 00:29:01,740
our laboratories and Providence Rhode

729
00:29:06,180 --> 00:29:04,909
Island doing some work to see if what

730
00:29:08,700 --> 00:29:06,190
brought

731
00:29:12,180 --> 00:29:08,710
saw in vitro in Virginia can reproduce

732
00:29:13,980 --> 00:29:12,190
can be reproduced in vivo will we really

733
00:29:20,070 --> 00:29:13,990
show that decrease in metastatic

734
00:29:22,409 --> 00:29:20,080
activity to round us all up resident

735
00:29:25,110 --> 00:29:22,419
molecular signaling is the platform that

736
00:29:27,419 --> 00:29:25,120
we've developed I'm confident that all

737
00:29:29,190 --> 00:29:27,429
we're doing is bridging biochemistry and

738
00:29:32,070 --> 00:29:29,200
biophysics we can explain everything we

739
00:29:32,639 --> 00:29:32,080
see biochemically but that's not the

740
00:29:34,980 --> 00:29:32,649
right answer

741
00:29:37,740 --> 00:29:34,990
there's more going at a different level

742
00:29:39,690 --> 00:29:37,750
which I'm calling biophysics and that

743
00:29:42,659 --> 00:29:39,700
gets us to this question of what's the

744
00:29:44,430 --> 00:29:42,669
nature of the signal the reason we're

745
00:29:46,440 --> 00:29:44,440
asking that question is not for academic

746
00:29:47,970 --> 00:29:46,450
purposes but we think that other

747
00:29:49,499 --> 00:29:47,980
modalities might be identified that

748
00:29:52,619 --> 00:29:49,509
could be more efficient than what we're

749
00:29:55,320 --> 00:29:52,629
doing maybe sound might be light mic the

750
00:29:57,029 --> 00:29:55,330
electromagnetic force any of those I

751
00:29:58,799 --> 00:29:57,039
have in parentheses bone healing they're

752
00:30:00,629 --> 00:29:58,809
just as kind of an aside to tell you

753
00:30:02,340 --> 00:30:00,639
that you all know that you can use

754
00:30:04,200 --> 00:30:02,350
magnets to stimulate bone healing right

755
00:30:05,940 --> 00:30:04,210
that's been in the literature hospitals

756
00:30:06,749 --> 00:30:05,950
use that all the time now we have a

757
00:30:08,759 --> 00:30:06,759
molecule

758
00:30:11,940 --> 00:30:08,769
it's called calmodulin that stimulates

759
00:30:14,820 --> 00:30:11,950
bone healing faster than magnet what's

760
00:30:18,299 --> 00:30:14,830
the common denominator between the

761
00:30:21,240 --> 00:30:18,309
calmodulin the biochemical approach and

762
00:30:22,139 --> 00:30:21,250
the magnet the biophysical we don't know

763
00:30:25,259 --> 00:30:22,149
we need help

764
00:30:32,490 --> 00:30:25,269
that's why I'm here and for that I thank

765
00:30:37,090 --> 00:30:34,810
looking for questions I imagine they'll

766
00:30:39,100 --> 00:30:37,100
be one or two I have to tell you that

767
00:30:42,100 --> 00:30:39,110
I've spoken at John's annual conference

768
00:30:44,649 --> 00:30:42,110
of his Beechtree labs and it's a mini

769
00:30:45,370 --> 00:30:44,659
version of sse then he calls the members

770
00:30:52,120 --> 00:30:45,380
beechnuts

771
00:30:56,169 --> 00:30:52,130
yeah thank you war that was really

772
00:31:00,279 --> 00:30:56,179
inspiring I understand that one of the

773
00:31:03,250 --> 00:31:00,289
reasons why spinal cord injuries after a

774
00:31:05,590 --> 00:31:03,260
period of time you know cannot be healed

775
00:31:07,299 --> 00:31:05,600
is because of scar tissue is there a

776
00:31:08,710 --> 00:31:07,309
possibility that this will work well

777
00:31:11,259 --> 00:31:08,720
that kind of thing if applied

778
00:31:15,370 --> 00:31:11,269
immediately after an injury it does work

779
00:31:17,289 --> 00:31:15,380
we've done it one of the Articles that I

780
00:31:19,539 --> 00:31:17,299
make my students undergraduate students

781
00:31:21,730 --> 00:31:19,549
read is as an article on use of stem

782
00:31:25,299 --> 00:31:21,740
cells and in that very injury in horses

783
00:31:27,930 --> 00:31:25,309
and it reported that there was very good

784
00:31:31,090 --> 00:31:27,940
good results from it have you looked at

785
00:31:33,720 --> 00:31:31,100
the comparison with the use of stem

786
00:31:36,970 --> 00:31:33,730
cells or using stem cells in in

787
00:31:38,409 --> 00:31:36,980
connection with slo we've not done any

788
00:31:39,610 --> 00:31:38,419
comparative work other than through the

789
00:31:40,930 --> 00:31:39,620
experiences of the attending

790
00:31:42,310 --> 00:31:40,940
veterinarians that are come from some of

791
00:31:46,629 --> 00:31:42,320
these really big clinics in the

792
00:31:48,700 --> 00:31:46,639
Lexington area and they're from their

793
00:31:52,210 --> 00:31:48,710
experiences their observations the

794
00:31:53,649 --> 00:31:52,220
results are pretty much similar the they

795
00:31:58,480 --> 00:31:53,659
like the SLO because it's easier and

796
00:32:00,310 --> 00:31:58,490
cheaper John I'm wondering if you've

797
00:32:02,889 --> 00:32:00,320
tried you mentioned horses I'm wondering

798
00:32:04,810 --> 00:32:02,899
if you have tried anything with dairy

799
00:32:06,879 --> 00:32:04,820
cattle in particular mastitis or bangs

800
00:32:11,019 --> 00:32:06,889
disease you know nothing with bangs lots

801
00:32:12,759 --> 00:32:11,029
with staph aureus induced mastitis and I

802
00:32:14,259 --> 00:32:12,769
would say our success has been limited

803
00:32:17,649 --> 00:32:14,269
primarily because the farmers don't want

804
00:32:19,240 --> 00:32:17,659
to keep up the therapy but we have had

805
00:32:20,680 --> 00:32:19,250
some degree of success we have not done

806
00:32:22,240 --> 00:32:20,690
a real trial with it we have

807
00:32:24,399 --> 00:32:22,250
veterinarians in our group who do use it

808
00:32:26,250 --> 00:32:24,409
routinely and finally I think I can help

809
00:32:29,759 --> 00:32:26,260
you get some of your answers I

810
00:32:31,810 --> 00:32:29,769
appreciate it thank you I'm just on

811
00:32:35,379 --> 00:32:31,820
theory behind all this have you ever

812
00:32:39,279 --> 00:32:35,389
encountered papers on non-contact

813
00:32:40,870 --> 00:32:39,289
friction no sound familiar you know this

814
00:32:42,970 --> 00:32:40,880
I think this you're on the bottom floor

815
00:32:44,350 --> 00:32:42,980
of a whole field of science that any

816
00:32:46,930 --> 00:32:44,360
animal molecule

817
00:32:49,570 --> 00:32:46,940
has a intense virtual photon field

818
00:32:51,280 --> 00:32:49,580
around it that's sort of like not not

819
00:32:53,830 --> 00:32:51,290
recognised by physics was just starting

820
00:32:56,370 --> 00:32:53,840
to to become that and that non-contact

821
00:32:59,110 --> 00:32:56,380
friction is that separate molecules

822
00:33:01,870 --> 00:32:59,120
hundred animals apart in nanometers

823
00:33:04,780 --> 00:33:01,880
apart have very strong friction between

824
00:33:06,760 --> 00:33:04,790
them for like AFM microscope stuff I

825
00:33:09,100 --> 00:33:06,770
would love to be introduced to it but

826
00:33:12,760 --> 00:33:09,110
look up non-contact friction will do it

827
00:33:13,560 --> 00:33:12,770
thank you I have a question and a

828
00:33:17,250 --> 00:33:13,570
comment

829
00:33:20,650 --> 00:33:17,260
I'm I'm interested in the dose response

830
00:33:22,180 --> 00:33:20,660
efficacy because if this is a molecular

831
00:33:24,940 --> 00:33:22,190
signaling kind of effect you might

832
00:33:27,820 --> 00:33:24,950
expect it to be those independent but I

833
00:33:31,510 --> 00:33:27,830
saw your dose response curve and in the

834
00:33:34,360 --> 00:33:31,520
last graph kind of indicating that it's

835
00:33:36,039 --> 00:33:34,370
acting like a ordinary biochemical kind

836
00:33:38,830 --> 00:33:36,049
of that's the first time we've ever seen

837
00:33:41,830 --> 00:33:38,840
anything like that so that's alone so

838
00:33:43,030 --> 00:33:41,840
nonetheless I wonder if you had done any

839
00:33:47,320 --> 00:33:43,040
experiments going the other direction

840
00:33:48,970 --> 00:33:47,330
like toward the ultra low light oceans

841
00:33:51,370 --> 00:33:48,980
we've done lots of that with the idea of

842
00:33:53,890 --> 00:33:51,380
hormesis involved yes right absolutely

843
00:33:55,960 --> 00:33:53,900
we have and it works at ultra low doses

844
00:33:57,909 --> 00:33:55,970
actually these are these are relatively

845
00:33:59,440 --> 00:33:57,919
ultra low doses and we get the classic

846
00:34:01,659 --> 00:33:59,450
u-shaped or J shaped curve that's

847
00:34:03,039 --> 00:34:01,669
associated with hormesis and these doses

848
00:34:05,620 --> 00:34:03,049
that I'm talking about are at the bottom

849
00:34:07,990 --> 00:34:05,630
of that you okay okay so that's they are

850
00:34:10,240 --> 00:34:08,000
it a higher dose clinically a higher

851
00:34:11,790 --> 00:34:10,250
dose will give us no response and a

852
00:34:14,169 --> 00:34:11,800
lower dose will give us no response

853
00:34:16,570 --> 00:34:14,179
there is a window with that new shape

854
00:34:19,060 --> 00:34:16,580
okay so that's encouraging and as far as

855
00:34:21,340 --> 00:34:19,070
testing hypothesis that there's some

856
00:34:23,349 --> 00:34:21,350
endogenous electromagnetic field which

857
00:34:27,099 --> 00:34:23,359
might carry the information like my

858
00:34:29,710 --> 00:34:27,109
laser last night I you could do

859
00:34:31,780 --> 00:34:29,720
experiments with looking for synergism

860
00:34:34,300 --> 00:34:31,790
between the chemical and an

861
00:34:36,450 --> 00:34:34,310
electromagnet exogenous electromagnetic

862
00:34:38,649 --> 00:34:36,460
field to see if there's any coupling or

863
00:34:39,909 --> 00:34:38,659
synergism between the two that's where

864
00:34:43,690 --> 00:34:39,919
we need help because we're too dumb to

865
00:34:47,560 --> 00:34:43,700
do those sorts of experiments Thank You

866
00:34:56,350 --> 00:34:53,830
bill didn't see you behind me thanks

867
00:34:59,590 --> 00:34:56,360
John I was wondering if you also have

868
00:35:02,440 --> 00:34:59,600
looked for a dose-response curve the

869
00:35:05,590 --> 00:35:02,450
u-shaped thing with respect to the

870
00:35:11,650 --> 00:35:05,600
frequency of repetition of the dose yes

871
00:35:13,750 --> 00:35:11,660
and it's interesting you ask the we can

872
00:35:16,510 --> 00:35:13,760
use a light switch as an example of what

873
00:35:17,950 --> 00:35:16,520
happens and all that you can you can

874
00:35:19,000 --> 00:35:17,960
turn a light switch on gently or can

875
00:35:20,980 --> 00:35:19,010
smash the heck out of it but you don't

876
00:35:23,620 --> 00:35:20,990
get any different response right either

877
00:35:28,630 --> 00:35:23,630
turns on or it doesn't what we find is

878
00:35:32,950 --> 00:35:28,640
that especially in acute diseases if we

879
00:35:35,620 --> 00:35:32,960
in a sense pulse therapy we get a better

880
00:35:37,000 --> 00:35:35,630
result early on and then we can reduce

881
00:35:38,680 --> 00:35:37,010
the frequency of administration but we

882
00:35:41,470 --> 00:35:38,690
keep the dose the same so it's like

883
00:35:43,170 --> 00:35:41,480
flicking the light switch on and off we

884
00:35:47,140 --> 00:35:43,180
think it's more important to have

885
00:35:51,880 --> 00:35:47,150
multiple endpoints in inputs than one

886
00:35:53,350 --> 00:35:51,890
big one and so yes and for example with

887
00:35:56,020 --> 00:35:53,360
rheumatoid arthritis we have a

888
00:35:57,340 --> 00:35:56,030
formulation that we use for that person

889
00:35:59,290 --> 00:35:57,350
is an extreme pain they're still going

890
00:36:01,690 --> 00:35:59,300
to take sublingual drops we would ask

891
00:36:03,670 --> 00:36:01,700
them to take a sublingual drop every 15

892
00:36:06,160 --> 00:36:03,680
minutes for an hour users in the hour

893
00:36:07,750 --> 00:36:06,170
that pain starts to resolve after they

894
00:36:10,480 --> 00:36:07,760
get over that threshold where pain

895
00:36:12,370 --> 00:36:10,490
reduction has begun they can decrease

896
00:36:15,460 --> 00:36:12,380
the frequency of drop maintain the same

897
00:36:17,500 --> 00:36:15,470
concentration and they'll be fine if

898
00:36:19,300 --> 00:36:17,510
they don't decrease the frequency

899
00:36:21,550 --> 00:36:19,310
it won't hurt them anything just wasted

900
00:36:22,930 --> 00:36:21,560
drops oh sorry it's just wasted drops

901
00:36:26,130 --> 00:36:22,940
you know it doesn't hurt them any so it

902
00:36:29,010 --> 00:36:26,140
doesn't doesn't overdose them okay nope